Con A Sepharose 4B - Names and Identifiers
Con A Sepharose 4B - Physico-chemical Properties
Solubility | Density of ligand: 5~10mg Con A/ml medium |
Appearance | Containing 20% ethanol, milky white colloid at the bottom |
Storage Condition | 2-8℃ |
Use | Separation and purification of sugars, glycoproteins, glycolipids, etc. with mannitol and glucose residues |
Con A Sepharose 4B - Introduction
Con A Sepharose 4B is a commonly used affinity chromatography medium, which is immobilized by Concanavalin A (Con A) and Sepharose 4B.
The main properties of Con A Sepharose 4B include:
-High affinity: Con A can efficiently bind to polysaccharides, glycoproteins and carbohydrate structures with residues such as α-D-mannose, α-D-glucose and α-D-galactose;
-Medium particle size: The particle size of Con A Sepharose 4B is generally between 45-165 microns;
-Alkali resistance: it remains stable in the pH range of 2-12;
-Antitoxic: Con A Sepharose 4B can be sterilized by solution filtration, UV irradiation or chemical disinfection.
Key uses of Con A Sepharose 4B include:
-affinity chromatography: purification and separation of target molecules by binding between Con A and specific polysaccharide, glycoprotein or carbohydrate structures;
-Structural analysis: used to identify and study carbohydrate structure and glycoprotein modification;
-Diagnostic tool: can be used to detect and quantify changes in carbohydrate or glycoprotein levels.
Con A Sepharose 4B is generally prepared by cross-linking Con A with Sepharose 4B and covalently immobilizing it on the particle surface.
For safety information, Con A Sepharose 4B is generally stable and safe. However, the product instructions should be read carefully before use and the relevant laboratory safety practices should be followed. Avoid direct contact with skin and eyes, such as accidental contact should immediately rinse with plenty of water. At the same time, ensure that the waste is disposed of in the proper pores and in accordance with local regulations.
Last Update:2024-04-09 21:11:58